Control of Lipase Content in Mucor Rennet Preparations

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Lipase of Mucor pusillus.

Lipase of Mucor pusillus NRRL 2543 was recovered with ammonium sulfate precipitation, gel filtration on Sephadex G-75, and anion-exchange chromatography on diethylaminoethyl-Sephadex A-50. Maximal glycerol ester hydrolase (lipase) activity was observed at pH 5.0 to 5.5 and 50 C when trioctanoin and olive oil were used as substrates. The enzyme also showed esterase activity; it hydrolyzed, with ...

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ژورنال

عنوان ژورنال: Journal of Dairy Science

سال: 1974

ISSN: 0022-0302

DOI: 10.3168/jds.s0022-0302(74)84984-2